Figure 1. Timeline of repeated DT injections.

CD11c-DTR mice received adoptive transfer of OT-II T cells (2×107 cells/ 300 μL of sterile PBS) I.V. via tail vein or penile vein injection. Control mice did not receive any cells. Group 1 mice received 1x DT injection on day 1; group 2 mice received 2x DT injections on days 1 and 3, and Group 3 mice received 3x DT injections on days 1, 3 and 5. All DT injections were given I.P. at 4ng/g of body weight.