Fig. 2.

ICBs therapy upregulates CD73 expression in iCCA cells through TNF-α/NF-κB signaling pathway. (A) Evaluation of the effect of TNF-α and IFN-γ on CD73 mRNA expression in human iCCA cells CCLP1 and 9810 as determined by RT-PCR assays. (B) Evaluation of the effect of IFN-γ on CD73 protein expression in iCCA cells as detected by WB assays. (C) WB assays evaluating the effect of different concentrations of TNF-α on CD73 protein expression, as well as the downstream NF-κB signaling pathway in iCCA cells. (D) WB assays evaluating the effect of TNF-α on CD73 expression and NF-κB signaling in iCCA cells, with or without NF-κB pathway inhibitor BAY11-7082. WB, western blot; *P < 0.05, **P < 0.01, ***P < 0.001