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Predominance of the SAα2,3Gal linkage as detected by lectin staining in horse trachea. The MAA lectin specific for SAα2,3Gal (α2-3; detected with fluorescein isothiocyanate (FITC)-labeled anti-DIG antibody) bound to horse and pig tracheal epithelium, whereas SNA lectin specific for SAα2,6Gal (α2-6; detected with rhodamine-labeled anti-DIG antibody) bound only to the latter. Blue staining is a nonspecific reaction.
