FIG. 5.

Binding reactivity of influenza A viruses with gangliosides of human and equine influenza viruses. Gangliosides (1 μmol), represented by II⁶(NeuAc)LacCer (NeuAcα2,6Gal), II³(NeuAc)LacCer (NeuAcα2,3Gal), and II³(NeuGc)LacCer (NeuGcα2,3Gal), were developed in thin-layer chromatography plates. The plates were incubated with virus (2⁸ hemagglutination units at 4°C for 9 h and then processed as described in Materials and Methods. Relative binding reactivity is shown, with the highest activity set at 100%.