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. 2024 Jul 1;15:1419787. doi: 10.3389/fimmu.2024.1419787

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Copyright © 2024 Cabrera, Tietäväinen, Jokiranta, Mäkelä, Vaheri, Mustonen, Vapalahti, Kanerva and Strandin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic analysis reveals distinct characteristics of PUUV LDGs and PMNs. (A) UMAP visualization of single live CD56^-/CD3^-/CD19^-/CD14^-/CD15⁺/CD66b⁺ cells, displaying LDGs (in blue) and PMNs (in red) in a concatenated format, enabling an in-depth examination of their phenotypic differences. (B) Multigraph color mapping depicting each fluorochrome’s Mean Fluorescence Intensity (MFI) for selected markers, including CD66b, CD11b, LOX-1, CD16, CD15, HLA-DR, and CD33. (C) Bar graph illustrating the proportion of PMNs and LDGs expressing CD16.