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. 2024 Jul 1;15:1397049. doi: 10.3389/fphys.2024.1397049

TABLE 1.

List of mouse qPCR primers and sequences. Epo, erythropoietin; Vegf, vascular endothelial growth factor; Hif, hypoxia inducible factor; Hmox-1, heme oxygenase 1; Pgk-1, phosphoglycerate kinase 1; Glut-1, glucose transporter 1; Cas8, caspase 8; αMhc, alpha myosin heavy chain; βMhc, beta myosin heavy chain; Anp, atrial natriuretic peptide, Bnp, brain natriuretic peptide; Eef1e1, Eukaryotic Translation Elongation Factor 1 Epsilon 1; Rpl32, ribosomal protein L32; β-Actin, beta-actin.

Target Primer Name Sequence (5′-3′) Annealing temperature (°C)
Epo EPO-F CAT​CTG​CGA​CAG​TCG​AGT​TCT​G 58
EPO-R CAC​AAC​CCA​TCG​TGA​CAT​TTT​C 58
Vegfa VEGF-A-F GGA​GAC​TCT​TCG​AGG​AGC​ACT​T 60
VEGF-A-R GGC​GAT​TTA​GCA​GCA​GAT​ATA​AGA​A 60
Vegfb VEGF-B-F TCT​GAG​CAT​GGA​ACT​CAT​GG 60
VEGF-B-R TCT​GCA​TTC​ACA​TTG​GCT​GT 60
Vegfr1 VEGFR-1-F TGG​ACC​CAG​ATG​AAG​TTC​CC 60
VEGFR-1-R GCG​ATT​TGC​CTA​GTT​TCA​GTC​T 60
Vegfr2 VEGFR-2-F GAG​AGC​AAG​GCG​CTG​CTA​GC 60
VEGFR-2-R GAC​AGA​GGC​GAT​GAA​TGG​TG 60
Vegfr3 VEGFR-3-F CTG​GCA​AAT​GGT​TAC​TCC​ATG​A 60
VEGFR-3-R ACA​ACC​CGT​GTG​TCT​TCA​CTG 60
Hif1α HIF-1α-F CCC​ATT​CCT​CAT​CCG​TCA​AAT​A 60
HIF-1α-R TTACGCATGGCCGTTTCT 60
Hif2α HIF-2α-F CAG​CTT​CCT​TCG​GAC​ACA​TAA 60
HIF-2α-R CTC​CAA​GGC​TTT​CAG​GTA​CAA 60
Hmox-1 HO-1-F GGT​GAT​GGC​TTC​CTT​GTA​CC 60
HO-1-R AGT​GAG​GCC​CAT​ACC​AGA​AG 60
Pgk-1 PGK-1-F CAC​AGA​AGG​CTG​GTG​GAT​TT 60
PGK-1-R CTT​TAG​CGC​CTC​CCA​AGA​TAG 60
Glut-1 GLUT-1-F GGT​GTG​CAG​CAG​CCT​GTG​TAC​G 60
GLUT-1-R TAG​GAC​ATC​CAA​GGC​AGC​CGT​TC 60
Cas-8 Cas-8-F TGC​TTG​GAC​TAC​ATC​CCA​CAC 60
Cas-8-R TGC​AGT​CTA​GGA​AGT​TGA​CCA 60
αMhc αMHC-F CAC​CAA​CAA​CCC​ATA​CGA​CTA​C 60
αMHC-R TCA​GCA​CAT​CAA​AGG​CAC​TAT​C 60
βMhc βMHC-F AGA​TGG​CTG​GTT​TGG​ATG​AG 60
βMHC-R TTG​GCC​TTG​GTC​AGA​GTA​TTG 60
Anp ANP-F GGG​TAG​GAT​TGA​CAG​GAT​TGG 60
ANP-R TTC​CTC​CTT​GGC​TGT​TAT​CTT​C 60
Bnp BNP-F GGG​AGA​ACA​CGG​CAT​CAT​T 60
BNP-R CCC​AGC​GGT​GAC​AGA​TAA​AG 60
Eef1e1 Eef1e1-F TAA​CAT​CAC​CCT​GGC​GGA​CA 60
Eef1e1-R TGA​CAA​AAC​CAG​CGA​GAC​ACA 60
Rpl32 Rpl32-F GCC​TCT​GGT​GAA​GCC​CAA​G 60
Rpl32-R TTG​TTG​CTC​CCA​TAA​CCG​ATG​T 60
β-Actin β-Actin-F TGT​GAT​GGT​GGG​AAT​GGG​TCA​GAA 60
β-Actin-R TGT​GGT​GCC​AGA​TCT​TCT​CCA​TGT 60