Table 1. Antibody panel used in this protocol.
Antibody | Fluorophore | Clone | Host | Company | Catalog number | Dilution (per 1 × 106 cells) |
Rhodopsin | Unconjugated | RET-P1 | Mouse | Invitrogen | MAS-11741 | 3 μL |
Zenon kit | Alexa-405 | - | - | Invitrogen | Z25013 | - |
Thy 1.2 | APC-eFluor-780 | 53-2.1 | Rat | eBioscience | 47-0902-82 | 1 μL |
Vimentin | Biotinylated | 280618 | Rat | R&D Systems | BAM2105 | 2 μL |
Streptavidin | BUV-395 | - | - | BD Biosciences | BD 564176 | 2 μL |
Ki67 | BUV-737 | 20Raj1 | Mouse | eBioscience | 367-5699-42 | 5 μL |
BrdU | PerCP-eFluor-710 | BU20A | Mouse | eBioscience | 46-5071-42 | 1 μL |