Table 3. Experimental groups used in this protocol.

Experimental groups	# retinae (per tube)	Approximate cell count	Cell type	Stain
Untreated/unstained	4	~2 M	-	-
DMSO control	4	~2 M	Müller glia, retinal ganglion cells, rod photoreceptors, proliferating cells, mitotically active cells	Vimentin_Strep-BUV-395, Thy1.2_APC-eFluor-780, Rho_Alexa-405, Ki67_BUV-737, BrdU_PerCP-eFluor-710
PNU-282987 treated	4	~2 M	Müller glia, retinal ganglion cells, rod photoreceptors, proliferating cells, mitotically active cells	Vimentin_Strep-BUV-395, Thy1.2_APC-eFluor-780, Rho_Alexa-405, Ki67_BUV-737, BrdU_PerCP-eFluor-710