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Expression of vFLIP in different cell lines. Total RNA was used for an ORF71-specific RT-PCR. The middle panel shows the transcripts detected from infected OMKs or transformed human and simian T cells. The bottom panel shows the corresponding RNA as detected by RT-PCR for β-actin. +RT, RT-PCR from first-strand cDNA; −RT, PCR from parallel control reaction, where Superscript reverse transcriptase was omitted. The locations of primers are indicated.
