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. 2024 Jun 27;13(6):999–1015. doi: 10.21037/gs-24-157

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2024 Gland Surgery. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Experimental validation of SPAG6 expression. (A) Multiple immunofluorescence staining of SPAG6 in pan-cancer tissue sections. Views of cancers, including high expression (LIHC, CHOL, and OV) and low expression (UCEC, CESC, and THCA) under microscopy (200×). SPAG6 = red, cellular nuclei = blue (DAPI). (B) Cell Counting Kit 8 assay of SPAG6. (C) Transwell migration assay of SPAG6 (10× magnification). The cells were fixed with 4% paraformaldehyde and stained with 0.1% crystal violet. Migrated cells were photographed with a microscope. ***, P<0.001. LIHC, liver hepatocellular carcinoma; CHOL, cholangiocarcinoma; OV, ovarian serous cystadenocarcinoma; UCEC, uterine corpus endometrial carcinoma; CESC, cervical squamous cell carcinoma and endocervical adenocarcinoma; THCA, thyroid carcinoma; DAPI, 4,6-diamidino-2-phenyiindole 2 hci.