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. Author manuscript; available in PMC: 2024 Jul 15.
Published in final edited form as: Cell Rep. 2024 Apr 27;43(5):114160. doi: 10.1016/j.celrep.2024.114160

Figure 5. FA contribution to metabolism but not viability, Ca2+ transient, or impedance.

Figure 5.

(A) MMb, MMs, and MMc formulations with FAs, without FAs (no FA), and chemically defined (CD).

(B) Viability of hiPSC-CMs cultured in MMb, MMs, or MMc formulations ± FA and CD from day 20 to day 33 (n = 4).

(C) Representative spontaneous Ca2+ transient recordings of hiPSC-CMs cultured in MMb, MMs, or MMc formulations ± FA and CD from day 20 to day 35.

(D) CTD75 of hiPSC-CMs cultured in MMb, MMs, or MMc formulations ± FA and CD (n = 8).

(E) Representative paced (0.8 Hz) impedance recordings of hiPSC-CMs cultured in MMb, MMs, or MMc formulations ± FA and CD from day 20 to day 32.

(F) Pulse width at 50% measured from impedance recordings of hiPSC-CMs cultured in MMb, MMs, or MMc formulations ± FA and CD (n = 4).

(G) OCR of hiPSC-CMs cultured in MMb, MMs, or MMc formulations ± FA and CD from day 20 to day 33 (n = 5).

(H) Basal respiration, maximal respiration, and spare respiratory capacity of hiPSC-CMs cultured in MMb, MMs, or MMc ± FA and CD (n = 5).

Each data point shape indicates a different hiPSC line. Data are presented as mean ± SEM. n = biological replicates, ANOVA, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005, ****p ≤ 0.0001. See also Figure S5.