Table 2.
microRNA primer sequences
| miRNA | Primer ID | Accession # | Primer sequence (5’- 3’) | Efficiency (%) |
|---|---|---|---|---|
| miR-21 | hsa-miR-21-5p | MIMATI0000076 | UAGCUUAUCAGACUGAUGUUGA | 101.3 |
| miR-155 | hsa-miR-155-5p | MIMAT0000646 | UUAAUGCUAAUCGUGAUAGGGGUU | 99.5 |
| miR-324 | hsa-miR-324-5p | MIMAT0000761 | CGCAUCCCCUAGGGCAUUGGUG | 101.1 |
| miR-346 | hsa-miR-346 | MIMAT0000826 | UGUCUGCCCGCAUGCCUGCCUCU | 100.3 |
| miR-93 | hsa-miR-93-5p | MIMAT0000093 | CAAAGUGCUGUUCGUGCAGGUAG | 100.3 |
| miR-132 | hsa-miR-132-3p | MIMAT0000426 | UAACAGUCUACAGCCAUGGUCG | 100.7 |
| miR-33b | hsa-miR-33b-5p | MIMAT0003301 | GUGCAUUGCUGUUGCAUUGC | 100.3 |
*miRNA primers were predesigned and validated by Qiagen. Primer efficiencies were tested in this study