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. 2024 Jul 15;25:185. doi: 10.1186/s13059-024-03307-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Establishment of DNA methylation at cattle CoRSIVs is particularly labile to periconceptional environment. A Graphic illustration of IVP/MOET experiments. B Overlap of differentially methylated regions (DMRs) between IVP and MOET offspring with CoRSIVs and control regions in three tissues: liver, muscle, and hypothalamus. C-E For the IVP vs. MOET experiments, volcano plots illustrating significant differences in average methylation between IVP and MOET at 217 CoRSIVs and matched control regions in (C) liver, (D) muscle, and (E) hypothalamus. Each point represents a CoRSIV or control region. P values are adjusted for multiple testing; significant p values are shown in pink