Figure 2. Overview of the pipeline and the methods used to assess the efficiency of recombination and mosaicism.

(a) In this study, we implemented a thorough characterization pipeline comprising several crucial steps. Our initial focus was on characterizing three unique global Cre-driver strains, achieved by mating female Cre-driver strains with male Rosa-floxed strains. The resulting F1 offspring were genotyped using either standard PCR or probe-based assays. The genetic material for these assays was derived from tail tips of E17.5 stage embryos or postnatal P1 to P3 pups. For tissue-specific analysis of recombination and mosaicism, we conducted histological and immunohistochemical analyses using whole embryos from E15.5 to E17.5 or P2 pups, obtained from the mating of female floxed and male tissue-specific Cre-strains. (b)The percentage of progeny recombination was determined by dividing the number of litters showing complete recombination by the total number of litters and multiplying it by 100. The percentages of progeny mosaicism and floxed patterns were determined in a similar fashion.