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. Author manuscript; available in PMC: 2024 Jul 15.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Jun 27;477(4):700–705. doi: 10.1016/j.bbrc.2016.06.122

Fig. 2. Hydrophobicity and conformational stability of Aβ40 fibrils and oligomers.

Fig. 2

(A) Hydrophobicity of the environment of Aβ40 fibrils (red), FOs (black) and PFOs (blue) was determined by monitoring the emission wavelength of acrylodan labels introduced at 13 positions along the Aβ sequence. (B) Conformational stability of Aβ40 fibrils (red), FOs (black) and PFOs (blue) was determined by following the C1/2 values for GdnSCN-mediated denaturation of acrylodan labels introduced at 13 positions along the Aβ sequence. (C) Denaturation of Aβ40 fibrils detected by fluorescence of thioflavin T (green), Sypro Orange (black) and acrylodan labels at positions 14 (blue) and 20 (red). (D) Denaturation of Aβ40 FOs detected by fluorescence of Sypro Orange (blue) and acrylodan labels at positions 14 (red) and 32 (black). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)