TABLE 2.

Sequences of oligonucleotide primers and probes used for consensus and for gamma-2 and betaherpesvirus-specific PCR for DNA polymerase and Southern hybridization

Oligonucleotide	Orientationb	5′→3′ sequencec
All herpesvirusesa
DFASA	+	GTG TTC GAC TTY GCN AGY YTN TAY CC
VYGA	+	ACG TGC AAC GCG GTG TAY GGN KTN ACN GG
GDTD1B	−	CGG CAT GCG ACA AAC ACG GAG TCN GTR TCN CCR TA
Specific primers
MndRHV1-p	−	CCT GTA GCG TGA CCG TCT CG
MndRHV2-p	−	CGT AAG ACT TTG ACA TCT CCA GC
MndCMV-p	−	CCA GGT TTT CTT CCA CGA AC
MndHVβ-p	−	AAC ACC TGA CGC CCC AAA GAT G
Specific probes
MndRHV1	+	GCG AAC ATG CTG CAG CGA CCC A
MndRHV2	+	AGA CGC GGC TCC GGC GCG CGG TAC G
MndCMV	+	TTA ATC GGG AGG ACT ACT CGG
MndHVβ	−	TGC TGT GTC AAT GTA ATC GGT G

^aDegenerate oligonucleotide primers described in reference 15. 

^b+, sense; −, antisense. 

^cPositions of degeneracy are given. N = A, C, G, and T; Y = C and T; R = A and G; K = G and T.