KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Rabbit anti-Pck1 | ThermoFisher Scientific | #720266; RRID: AB_2633227 |
| Rabbit anti-Gsta2 | ThermoFisher Scientific | #PA5-96757; RRID: AB_2808559 |
| Alexa Goat anti-rabbit IgG 594 | ThermoFisher Scientific | #A32740; RRID: AB_2762824 |
| Mouse monoclonal anti-human SPI1 | ThermoFisher Scientific | #H00006688-M02; RRID: AB_913827 |
| Rabbit monoclonal anti-human TREM2 | ThermoFisher Scientific | #702886; RRID: AB_2762383 |
| Rabbit anti-TREM2 | Proteintech | #13483-1 - AP; RRID: AB_2605803 |
| HRP-conjugated donkey anti-mouse IgG | Bethyl Laboratories | #A90-337P; RRID: AB_10631367 |
| HRP- conjugated donkey anti-rabbit IgG | Bethyl Laboratories | #A120-208P ; RRID: AB_66770 |
| CD163-AF647 | BioLegend | #333619; RRID: AB_2563474 |
| Goat anti-LYVE1 | R&D Systems | # AF2089; RRID: AB_355144 |
| Donkey anti-rabbit-AF555 | ThermoFisher Scientific | # A32794; RRID: AB_162543 |
| Donkey anti-goat AF488 | ThermoFisher Scientific | # A32814; RRID: AB_2534102 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| PBS | ThermoFisher Scientific | #AM9625 |
| BSA | Sigma | # A1595 |
| 4% PFA | ThermoFisher Scientific | #101176-014 |
| 10% formalin | VWR | #100503-120 |
| 0.5% periodic acid solution | Sigma | #1.00482 |
| Schiff reagent | Sigma | # 1.09033 |
| Mayer’s hematoxylin | Sigma | # 51275 |
| 5x SSCT | ThermoFisher Scientific | # 15557044 |
| Opal reagent 570 | Akoya Bio | # FP1488001KT |
| Opal reagent 650 | Akoya Bio | # FP1496001KT |
| Liberase TH | Roche Diagnostics | # 5401119001 |
| RPMI-1640 | ThermoFisher Scientific | # 11875119 |
| ACK lysing buffer | Thermo Fisher Scientific | # A1049201 |
| CellTrics filter | Sysmex America Inc | # 04-0042-2316 |
| Humulin N solution | Eli Lilly | #HI-310 |
|
| ||
| Critical commercial assays | ||
|
| ||
| Chromium Single Cell | 10x Genomics | https://www.10xgenomics.com/platforms/chromium |
|
| ||
| Deposited data | ||
|
| ||
| Mouse single cell data | Current study | GEO: GSE205594 |
| Human single cell data | Current study | Accession: SCP2188 |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| C57BL/6 | The Jackson Lab | IMSR_JAX:000664 |
| 129S1 | The Jackson Lab | IMSR_JAX:002448 |
| BTBR.Cg-Lepob/WiscJ | The Jackson Lab | IMSR_JAX:004824 |
| C57BL/6N-Trem2em1cyagen | Cyagen | KOCMP-83433-Trem2-B6NVA |
|
| ||
| Software and algorithms | ||
|
| ||
| Graphpad Prism | GraphPad Software | https://www.graphpad.com/ |
| ImageJ | ImageJ | https://imagej.net/software/imagej/ |
| Adobe Illustrator | Adobe | https://www.adobe.com/ |
| Preprocessing of single-cell RNAseq sequencing files | Cellranger toolkit (v6.0.2), Cellbender (v0.2.0) | N/A |
| Genome references | mm10 for mouse, GrCh38 for human | N/A |
| Analysis of single-cell RNAseq data using R packages | R (v4.0.3), Seurat (v4.1.1), ggplot2, cowplot, pheatmap, UpsetR, scDblFinder (v1.4.0), Harmony | N/A |
|
| ||
| Other | ||
|
| ||
| High-fat diet | Envigo | TD.93075 dough |
| Standard chow | LabDiet | PicoLab Rodent Diet 20 pellets |
| Glucose meter One-touch UltraMini | Lifescan | https://www.onetouch.com/ |