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. Author manuscript; available in PMC: 2024 Jul 15.
Published in final edited form as: J Am Chem Soc. 2023 Jun 16;145(25):14155–14163. doi: 10.1021/jacs.3c05159

Figure 7: The degradation assay of TCO-DT2216 with c(RGDyK)-Tz.

Figure 7:

A) The ‘click’ reaction between TCO-DT2216 and c(RGDyK)-Tz releases the activated DT2216. B) Western blot analysis of Bcl-XL protein levels from HeLa cells treated with the indicated doses of treatments for 16 h. C) Western blot analysis of Bcl-XL and GAPDH levels in HeLa cells after the co-treatment with 10 μM VHL ligand and crPROTAC (TCO-DT2216 + c(RGDyK)-Tz, 3 h) for 16 h. D) Western blot analysis of BRD4 and GAPDH levels in HeLa cells after the co-treatment with 5 μM MG-132 and crPROTAC (TCO-DT2216 + c(RGDyK)-Tz, 3 h) for 16 h.