FIG. 4.

Expression of TBE virus prM/E antigens. Cell cultures were infected with 0.1 (A) or 1.0 (B) PFU/cell, and total proteins were harvested 72 h postinfection. Lane 1, size marker (positions are indicated in kilodaltons); lanes 2 to 5, complementing cells (A; RK-D4R-44) or cells nonpermissive for defective virus (B; RK-13) infected with the defective virus clones vD4-prME#6 (lane 2) and vD4-prME#9 (lane 3), negative control defective virus vD4-vA (lane 4), and positive control wt virus varec280 (lane 5); lane 6, CV-1 cells infected with varec280; lane 7, purified TBE virus glycoprotein E. The upper arrow points to the prM/E fusion protein and the lower arrow points to glycoprotein E.