FIG. 2.

gB and gH are distributed to the same subcellular sites in HCMV-infected Caco-2. Caco-2 cell monolayers at a TER of 250 Ω · cm² were infected basolaterally at an MOI of 25. At day 16 p.i., monolayers were fixed and stained for the presence of gB (early) and gH (late) glycoproteins. Confocal microscopy demonstrated that gB and gH were colocalized. In permeabilized cells (A and B), the majority of gB (A) and gH (B) were observed to be colocalized intracellularly in an endoplasmic reticulum- or Golgi-like staining pattern. In nonpermeabilized cells (C and D), both gB (C) and gH (D) were observed to be localized to the apical surface of infected cells, although they were present in much lower concentrations on the cell surface than was observed intracellularly.