Basal expression of Trx and Bcl-2 in monocytes from HIV-1-infected patients. After cell permeabilization with methanol, whole-blood samples were incubated with anti-Bcl-2 or anti-Trx antibodies. The mean fluorescence intensity of anti-Bcl-2 and anti-Trx antibodies was recorded as described in Materials and Methods, and the mean fluorescence intensity of the isotypic control was subtracted. Values are means ± SEM. ∗, significantly different from control values (P < 0.05); ○, significantly different from groups 1 and 2 (P < 0.05); +, significantly different from group 3 (P < 0.05).