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Histopathology assessment of kidney tissue sections post-DESI-MSI. Hematoxylin and eosin (H&E) stain of MeOH control and post-DESI analyzed tissue highlighting protein fixation as an effect of the interaction of solvent with tissue proteins and intensified by the higher temperature from the heated transfer line (HTL). Periodic acid–Schiff’s (PAS) staining on the same sections highlighting intact basement membranes post-DESI-MSI submitted to both HTL temperatures
