Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 23;416(18):4015–4028. doi: 10.1007/s00216-024-05339-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Image analysis. a AI-enabled (HALO AI®) image analysis workflow of mIF data. b mIHC labelling of epithelial cells of the collecting ducts with pan-cytokeratin (PK, cyan chromogen) and glomerulus with vimentin and same region. c mIF composite image of podocytes (P57⁺, green labelling), PFKFB3⁺ (yellow labelling), and HIF1α⁺ (red labelling) cells (NucBlue™—nuclear marker). d Nuclear segmentation and PFKFB3⁺ cells identified by AI-enabled image analysis. Tissue was analyzed by DESI-MSI at 10 scans/s with the HTL at 450 °C and H&E-stained prior to mIF and mIHC