FIG. 3.

(a) Time-dependent T-cell proliferation stimulated by HTLV-1-infected DCs. Mature HTLV-1-infected DCs were generated by exposure of immature DCs to 20 CCID₅₀ of HTLV-1 and cocultured with unseparated T cells (10⁵/well) at a DC:T-cell ratio of 1:20 or 1:40 in triplicate. Mock-infected DCs were used as a negative control. (b) Ag dose-dependent proliferation of T cells. Immature DCs were pulsed with various doses of live HTLV-1, and unseparated or CD4⁺ T cells (5 × 10⁴/well) were cocultured with HTLV-1- or mock-infected DCs at a DC:T-cell ratio of 1:20 in triplicate.