FIG. 5.

Comparison of T-cell-stimulating activities of HTLV-1-infected DCs and inactivated virion-pulsed DCs. (a) Immature DCs were either infected with various doses of HTLV-1 or pulsed with an equivalent dose of heat-inactivated virions, subsequently matured by LPS, and cocultured with autologous unseparated, CD4⁺ and CD8⁺ T cells (10⁵/well) for 5 days at a DC:T-cell ratio of 1:20. (b) LPS-matured DCs were exposed to live or inactivated virus for 4 days and cocultured with various T cells (10⁵/well) for 5 days at a DC:T-cell ratio of 1:20. A representative result of three independent experiments is shown. Each experiment was performed in triplicate.