FIG. 7.

Autologous T-cell proliferation by DCs from HAM/TSP patients. DCs were generated from peripheral monocytes obtained from an uninfected donor and HAM/TSP patients. Plastic-adherent monocytes were obtained from the population of CD2⁺ T cells depleted in advance by using beads. CD4⁺ or CD8⁺ T cells (5 × 10⁴/well) were stimulated with 2.5 × 10³ autologous HTLV-1-infected, inactivated Ag-pulsed or unpulsed DCs per well for 5 days in triplicate. Mature DCs from HAM/TSP patient 2 expressed HTLV-1 Ag on the surface without any exposure to HTLV-1 Ag in vitro and those from patient 1 did not.