Figure 11.

Relationships of Cx36 and N-cadherin at morphologically mixed synapses on motoneurons in the spinal cord and on large neurons in the red nucleus. (A) Double immunofluorescence labelling of Cx36 and N-cadherin with blue fluorescence Nissl counterstain in the lumbar spinal cord ventral horn of adult rat, showing overlay image of Cx36-puncta associated with labelling of N-cadherin on the somata (arrow) and initial dendrites (arrowheads) of a large motoneuron (asterisk), with inset of boxed area showing labelling of Cx36 (red) and N-cadherin (green) (arrows) shown magnified in overlay (arrow). (B) Double immunofluorescence labelling of Cx36 and N-cadherin with blue fluorescence Nissl counterstain in the red nucleus of adult mouse, with overlay image showing distribution of Cx36-puncta with N-cadherin on the surface of large neuronal somata (large arrows). (C) Magnification of the single neuronal somata (asterisk) in the boxed area of (B) (rotated clockwise by 90 degrees), showing nearly all somal Cx36-puncta (C1, arrows) associated with labelling of N-cadherin (C2, arrows), as seen in overlay (C3, arrows), and separate labelling of N-cadherin devoid of association with Cx36 (arrowhead).