FIG. 1.
Blocking of HIV-1 replication by SDF-1. T1 CD4+ cells were pretreated for 2 h with 1 μg of the indicated chemokine/ml, acutely infected with HIV-1 IIIB at an MOI of 10−2, and cultured in 2 ml of medium as described in the text. At 2- to 4-day intervals, 1 ml of medium was removed for quantitative HIV-1 p24 antigen determination and replaced with fresh medium containing 1 μg of the appropriate chemokine/ml. HIV-1 p24 antigen concentrations were determined at the indicated days of culture.