(A) LYVE1 immunofluorescence in the kidneys of control, SSHTN, and SSHTN+AVE male mice. LYVE1 is labeled green and lymphatic vessels are indicated with arrows surrounding the blood vessels (BV). Images were taken in the cortex at 20×. Scale bars = 50 µm. (B) Renal lymphatic density in control, SSHTN, and SSHTN+AVE male mice as determined by quantification of LYVE1+ pixels per field. (C) Renal expression changes in lymphangiogenesis-related genes in control, SSHTN, and SSHTN+AVE male mice. Results are presented as mean ± SEM and all statistical analyses were performed with one-way ANOVA (n = 3-5 per group). *P<0.05 vs control mice and #P<0.05 vs SSHTN mice.