FIG. 1.

Construction of RV M33 virus full-length cDNA clone. The numbers on the viral genome scale refer to the distance from the 5′ end in kilobases. Six DNA fragments were amplified by proofreading TaKaRa Taq DNA polymerase with the requisite primers as described in Materials and Methods. The amplified DNA fragments were ligated into a full-length cDNA representing the whole viral genome by using the restriction sites indicated above the genome. The full-length cDNA was cut with EcoRI and HindIII and inserted into a modified pBR322 plasmid that had been cut with the same enzymes to obtain the full-length cDNA clone pBRM33.