TABLE 2.
Relative rates of synthesis and release of viral structural protein
| Virus | Relative rate of synthesis and release of:
|
|||
|---|---|---|---|---|
| Intracellular E1 protein
|
Extracelluar E1 protein
|
|||
| Radiolabela | Ratiob | Radiolabelc | Ratiob | |
| BRM34 | 10.6 | 1.0 | 5.2 | 1.0 |
| C466A/C467A | 6.2 | 0.6 | 1.6 | 0.3 |
| C467A | 7.8 | 0.7 | 2.3 | 0.4 |
| C470A | 10.2 | 1.0 | 5.6 | 1.0 |
| L471A | 8.6 | 0.8 | 3.5 | 0.7 |
| L464A | 10.8 | 1.0 | 4.7 | 0.9 |
| C466A | 10.9 | 1.0 | 4.3 | 0.8 |
a
Amounts of radiolabel (expressed in densitometry units) in intracellular E1 bands after 10 min of chase in Fig. 5A were quantitated by densitometry of autoradiographs.
b
Ratio of radiolabel to that in BRM34.
c
Amounts of radiolabel (expressed in densitometry units) in extracellular E1 bands in the virions released into medium after 5 h of chase (Fig. 5B) were quantitated by densitometry of autoradiographs.