Fig. 3. RAB32 S71R triggers increased LRRK2 kinase activity.
Western blot analyses of cotransfections in HEK293 cells, where Myc-LRRK2 WT was introduced alongside either HA-RAB32 WT or HA-RAB32 S71R. The bar plots show mean ± s.d. a, LRRK2 S1292 phosphorylation; n = 3 biological replicates are shown (two-tailed one-sample t-test, P = 0.02; Extended Data Fig. 9). b, LRRK2 and RAB32 coimmunoprecipitation; n = 3 biological replicates are shown (two-tailed one-sample t-test, P = 0.034; Extended Data Fig. 9). *P < 0.05. Full-length blots are provided as source data. Mut, mutant.