FIG. 3.
Binding to gp120 from divergent HIV-1 isolates by sera from rgp120SF2- and rgp120CM235-immunized baboons. Monomeric gp120 was captured from the supernatant of detergent-treated acutely infected cells. TCLA isolates from subtypes B (IIIB and SF2) and E (NPO3, 9466, 9461, and 42368) and primary isolates from subtypes B (US/660, SF13, US/717, and 9013) and E (9466, CM235, CMU06, and 8868) were evaluated. Data are presented as serum endpoint titer (determined by EIA) against each gp120 (A) and ratio of baboon serum binding to the HIV-1 serum pool from the same subtype (B). The endpoint titers and binding ratios are means of the 5 rgp120SF2 and 10 rgp120CM235 immune sera. Mean endpoint titers for individual preimmune baboon sera binding to each of the gp120 were <1:100 in panel A.