Table 2.
Sequences of the primers and probes used in the TLAMP assay.
| Primer-probe | Sequence (5′–3′) | TM (°C) |
|---|---|---|
| H5-F3 | TTCCATGACTCAAATGTCAAG | 57.6 |
| H5-B3 | GCTAGGGAACCCGCCACT | 59.7 |
| H5-FIP-Q | BHQ1-CATACATTCATTATCACATTTGTGGACTACAGCTTAGGGAYAATGCAAA b | 63.2/59.6 a |
| H5-BIP | GAAAGTGTGAGAAATGGGACGTTCTTCCCTTTTTARTCTTGCTT b | 60.5/55.7 a |
| H5-Floop | CGAAACAACCATTACCCAGCTC | 61.3 |
| H5-Bloop | ATGACTACCCCCAGTATTCAGAAG | 58.7 |
| H5-FD | ATGTGATAATGAATGTATG-FAM | 39.2 |
| H7-F3 | TCACATACAATGGAATAAGAAC | 55.2 |
| H7-B3 | CCCATATAGCTTGGTTTGCT | 57.2 |
| H7-FIP-Q | BHQ3-CTGTGTTTGACAGGAGCCATTTCGTGACCAGTGCATGTAGG | 56.5/61.9 a |
| H7-BIP | TTCCCGCAGATGACTAARTCATAAGTTGAAACGGAATGRTGGA b | 56.7/61.3 a |
| H7-Floop | TCTGCATAGAATGAAGATCCTGATC | 59.7 |
| H7-Bloop | CCAGCTATAGTAGTATGGGG | 57.8 |
| H7-FD | CTCCTGTCAAACACAG-Cy5 | 41.5 |
| H9-F3 | ACAAAATGAACAAGCAGTATGAAAT | 57.6 |
| H9-B3 | TCCATGCATTGGTCATCACATT | 60.8 |
| H9-FIP-Q | BHQ2-GATCGTCAATCTTATTGTTAATCATGATCATGAATTCAGCGAGG | 55.0/55.0 a |
| H9-BIP | TATGGGCATATAATGCAGARTTGCTTTTGCATCATGCTCATCGA b | 56.8/60.9 a |
| H9-Floop | TTGTTAATCATGTTAAGTCTAGTTT | 57.1 |
| H9-Bloop | AGAATTGCTAGTTCTGCTTGAAAA | 58.0 |
| H9-FD | AACAATAAGATTGACGATC-Cy3 | 44.0 |
a
The inner primer consists of two primers joined together, such as FIP=F1c + F2 and BIP=B1c + B2, so there are two annealing temperatures.
b
The bold font indicates degenerate sites (R: A/G; Y: C/T).