TABLE 1.

Primers used for TnSeq.

Name	Sequence (5'→3′)	Sample barcode	Description	Source
P5-IR2-1	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNAGgatcagTCAGGGTTGAGATGTGTATAAGAGACAG	DDW 1	Semi-arbitrary PCR method	Mandal et al. (2021)
P5-IR2-2	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNAGcttgtaTCAGGGTTGAGATGTGTATAAGAGACAG	L-Arginine 1	Semi-arbitrary PCR method	Mandal et al. (2021)
P5-IR2-3	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNAGataacgTCAGGGTTGAGATGTGTATAAGAGACAG	DDW 2	Semi-arbitrary PCR method	Mandal et al. (2021)
P5-IR2-4	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNAGggctagTCAGGGTTGAGATGTGTATAAGAGACAG	L-Arginine 2	Semi-arbitrary PCR method	Mandal et al. (2021)
P5-IR2-5	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNAGttcggcTCAGGGTTGAGATGTGTATAAGAGACAG	T0 1	Semi-arbitrary PCR method	Mandal et al. (2021)
P5-IR2-6	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNAGcagatcTCAGGGTTGAGATGTGTATAAGAGACAG	T0 2	Semi-arbitrary PCR method	Mandal et al. (2021)
Ez tn5 <KAN2> FP	ACC​TAC​AAC​AAA​GCT​CTC​ATC​AAC​C		Semi-arbitrary PCR method	EZ-Tn5™ <KAN-2>Tnp Transposome™ Kit
Rnd1-PE-arb1	CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTNNNNNNNNNNTGCTG		Semi-arbitrary PCR method	Custom primer based on (Christen et al., 2011) primer
round2-PE-P7	CAA​GCA​GAA​GAC​GGC​ATA​CGA​GAT​CGG​TCT​CGG​CAT​TCC​TGC​TGA​ACC​GCT​CTT​CCG​ATC​T		Semi-arbitrary PCR method	Christen et al. (2011)