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. 2024 Jul 16;7:865. doi: 10.1038/s42003-024-06529-3

Fig. 2. Characterization of AAV vector constructs.

Fig. 2

a Western blot analysis of supernatants of HEK293T cells transfected with the indicated AAV vector constructs stained against human IgG1. Supernatants of mock-transfected cells and purified TRES6hu antibody at the indicated amounts were included as negative and positive controls, respectively. b Tenfold serial dilutions of supernatants of cells transfected with the indicated AAV vector constructs were analysed by a flow cytometric binding assay for the SARS-CoV-2 spike protein (one representative is shown out of three experiments). c Anti-human IgG1 Western blot analysis of supernatants of HEK293T cells transduced with the indicated dose (vg copies per cell) of the AAV vector construct packaged in AAV2 capsids, 0.5 µg of purified TRES6hu antibody served as control. d Human iPSC-derived cardiomyocytes were transduced with the AAV-TRES6 vector construct packaged in AAVMYO capsids at the indicated dose. Mock transduced cells and 0.5 µg of purified TRES6 antibody served as controls. All uncropped blots are shown in Fig. S1.