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. 2024 Jul 16;7:865. doi: 10.1038/s42003-024-06529-3

Fig. 7. Fc glycosylation and impact on Fc-γ receptor interaction.

Fig. 7

a The composition of glycans attached to the amino acid N297 of the antibody Fc part was measured by mass spectrometry for AAV8 (red)- and AAVMYO (green)-delivered TRES6. The samples were obtained in week 4 (W4, filled) and week 12 (W12, shaded) during the long-term kinetic experiment. The binding of in vivo produced TRES6 (AAV8: red; AAVMYO: green) or TRE6 N297A (blue) to the murine Fc-γ receptors RI (b), RIIb (c), RIII (d), and RIV (e) was analysed. Sera from week 8 and week 52 during the long-term kinetic experiment were used and diluted to a TRES6 concentration of 100 ng/ml. Each bar shows the mean value of the group ± SEM. Statistical evaluation of the data were performed by Kruskal–Wallis test (one-way ANOVA) and Dunn’s pairwise multiple comparison procedures as post hoc test (*p ≤ 0.05; ***p ≤ 0.001; ****p ≤ 0.0001; not indicated: non-significant).