Figure 2.

Genetic deletion of Nedd4L results in the loss of quiescence-associated genes in MuSCs

(A) Schematic of the isolation of MuSCs and RNA-seq, generated with Biorender.com. n = 3 male mice per condition, aged 5–6 weeks.

(B) Heatmap of differentially expressed genes between WT and Nedd4L-cKO MuSCs. Threshold is adjusted p value ≤0.05, LFC ≥1, RPM ≥5.

(C) PCA plot of WT and Nedd4L-cKO MuSCs.

(D) Pearson correlation of the WT and Nedd4L-cKO MuSCs.

(E) Volcano plot of WT vs. Nedd4L-cKO transcripts.

(F) Bar graph of the absolute expression (RPM) of Nr1d1 in WT and Nedd4L-cKO MuSCs and in WT myoblasts. n = 3; two-tailed t test, data presented as mean ± SD.

(G) Bar graph of the absolute expression (RPM) of Chrdl2 in WT and Nedd4L-cKO MuSCs and in WT myoblasts. n = 3; two-tailed t test, data presented as mean ± SD.

(H) Bar graph of the absolute expression (RPM) of Calcr in WT and Nedd4L-cKO. n = 3; two-tailed t test, data presented as mean ± SD.

(I) Immunofluorescence of Pax7 and Chrdl2 in MuSCs associated to freshly isolated EDL myofibers. Scale bar: 25 μm.