Figure 3.

Genetic deletion of Nedd4L upregulates pathways associated with activation and differentiation

(A) Top 20 upregulated Reactome pathways in Nedd4L-cKO MuSCs.

(B) All downregulated Reactome pathways in Nedd4L-cKO MuSCs.

(C) Selected enrichment plots for the translation, respiratory electron transport, cell-cycle checkpoints, and muscle contraction pathways.

(D) Heatmap of the expression of the mitochondrial genes in WT and Nedd4L-cKO MuSCs.

(E) Bar graph of the percentage of mitochondrial reads from the RNA-seq of WT and Nedd4L-cKO MuSCs, with the quiescent and activated MuSC RNA-seq from publicly available data retrieved from the GEO GSE144871.¹⁴