Figure 5.

Genetic deletion of Nedd4L alters the proteome of freshly isolated MuSCs

(A) Diagram of the design of the mass spectrometry experiment performed on 200,000 freshly isolated WT and Nedd4L-cKO MuSCs, generated with Biorender.com. Mice were pooled to obtain enough MuSCs, n = 7 WT, and 10 knockout mice.

(B) Heatmap of all detected proteins in 200,000 freshly isolated MuSCs from WT and Nedd4L-cKO mice.

(C) Heatmap of the protein levels in WT and Nedd4L-cKO MuSCs for all significantly different proteins (LFC ≥2, p value ≤0.05).

(D) PCA plot of WT and Nedd4L-cKO MuSCs.

(E) Bar graph representing the DCX protein levels from the Mass spec. n = 4; two-tailed t test, data presented as mean ± SD.

(F) IGV tracks of RNA-seq reads for Dcx.

(G) Bar graph of the absolute expression (RPM) of Dcx in WT and Nedd4L-cKO mice. n = 4 male mice; two-tailed t test, data presented as mean ± SD.

(H) Immunofluorescence for DCX and PAX7 in WT MuSCs associated with freshly isolated (0 h) EDL myofibers.

(I) Immunofluorescence for DCX and PAX7 in Nedd4L-cKO MuSCs associated with freshly isolated EDL myofibers.

(J) Bar graph representing the percentage of Dcx⁺/Pax7⁺ MuSCs associated to freshly isolated EDL myofibers. n = 4; two-tailed t test, data presented as mean ± SD.