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Plasmid-based assays show effects of the L1 insertion on AR expression in HEK293 cells. (A) constructs created for individual transfections, and experimental workflow. (B) AR mRNA levels normalized to neomycin (which is encoded by the plasmid). (C) AR protein levels in HEK293 transfected with the individual constructs, either with (+) or without (−) treatment with dihydrotestosterone (DHT). Tubulin served as a loading control. WB: Western Blot.
