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. 2024 Mar 5;49(9):1392–1401. doi: 10.1038/s41386-024-01835-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Exemplary images of DAPI (blue) and MET-GFP (green) in layers 5 and 6 mPFC at P35 (left) and P90 (right). Blue arrows denote DAPI nuclei, yellow arrows denote GFP⁺ cells, scale bars = 50 µm. B Quantification of the percentage of MET-GFP⁺ cells in layer 5 mPFC at P35 (n = 5) and P90 (n = 6). No significant difference between ages was determined. C Quantification of the percentage of MET-GFP⁺ cells in layer 6 mPFC at P35 (n = 6) and P90 (n = 6). No significant difference between ages was determined. D Quantification of the percentage time freezing of Met^GFP Shock mice conditioned on P35 (n = 6) or P90 (n = 6) and tested 1 d later. No significant difference between ages was determined. E Quantification of the percentage of FOS⁺ cells in layer 5 mPFC in P35 and P90 home-caged and contextual fear memory formation tested mice (P35 home-caged: n = 5; P35 memory: n = 6; P90 home-caged: n = 6; P90 memory: n = 6). There were significant age and condition effects, with a significant difference between P90 home-caged and P90 memory tested mice (*p < 0.05, **p < 0.01, ns, not significant). F Quantification of the percentage of FOS⁺ cells in layer 6 mPFC in P35 and P90 home-caged and contextual fear memory formation tested mice (P35 home-caged: n = 6; P35 memory: n = 6; P90 home-caged: n = 6; P90 memory: n = 6). There was a significant condition effect, with a significant difference between P90 home-caged and P90 memory tested mice (**p < 0.01, ns, not significant). G Exemplary images of FOS (magenta) and MET-GFP (green) in layers 5 and 6 mPFC at P35 (left) and P90 (right). Blue arrows denote FOS⁺ cells, yellow arrows denote FOS⁺;GFP⁺ colocalized cells, scale bars = 50 µm. H Quantification of the percentage of FOS⁺;GFP⁺ colocalized cells out of total FOS-expressing cells in layer 5 mPFC in mice conditioned on P35 (n = 6) or P90 (n = 6) and tested 1 d later and in layer 6 mPFC I. There was a significant difference between ages in both layers (**p < 0.01).