Skip to main content
. 2024 Jun 5;43(14):2979–3008. doi: 10.1038/s44318-024-00132-2

Figure EV4. Activities of respiratory complexes III, IV, and V of CL-binding mutants.

Figure EV4

(A) Complex III activity in 0.5% (w/v) DDM-solubilized mitochondria (n = 4–6, biological replicates). (B) Complex IV activity in 0.5% (w/v) DDM-solubilized mitochondria (n = 6–9, biological replicates). (C) Complex V in-gel activity assay. Mitochondria were solubilized in 1% (w/v) DDM, resolved by 5–12% blue native-PAGE, and incubated with the substrate (n = 5, biological replicates). Mean with SEM. Significant differences obtained by two-way ANOVA followed by Tukey’s multiple comparisons test are shown as * for comparison with WT and † for comparison between pockets; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.