FIG. 1.
(A). Schematic depiction of partial HIV-1 Gag and Pol sequences of the p6Gag mutant (HXB2Pro) and parental construct (HXB2) used in this study. Proline residues at positions 5 and 7 of p6Gag where changed to arginine and glutamine (boldface), respectively, without altering the Pol amino acid sequence. (B to E) Replication growth curve of HXB2 and HXB2Pro viruses as monitored by RT activity from cell-free supernatants. Virions were generated from transfected COS-7 cells and used to initiate infections in Jurkat (B), CEM (C), or H9 (D) cells or PBMC (E). PBMC were additionally infected with a 1,000-fold-reduced concentration of the HXB2 virus [HXB2(1/1000)], to check the relative degree of infectivity of the HXB2Pro virus.