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Radioimmunoprecipitation of viral proteins from uninfected H9 (Mock) cells, established H9 cell lines expressing p6Gag mutant (HXB2ProBgl), and parental (HXB2Bgl) constructs. The cells were metabolically labeled with [35S]cysteine for 12 h. The proteins were immunoprecipitated with HIV-1-positive patient serum either from cell lysates (A) or from particles pelleted through 20% sucrose (B). The proteins were separated by SDS-PAGE and visualized by autoradiography.
