FIG. 5.

Quantitation of virion-associated protein profiles derived from Jurkat (A and B) and H9 (C and D) cell lines. Twofold dilutions of HXB2Bgl virion lysates (as indicated below each corresponding lane) were run side by side with a fixed amount of HXB2ProBgl (furthest-right lane) to compare the relative amounts of Gag proteins and RT proteins in each sample. Virion proteins from mock-infected and HXB2Bgl- and HXB2ProBgl-infected cells were separated by 12% polyacrylamide gels and transferred onto nitrocellulose membranes. The blots were reacted with either HIV-1-positive human serum (A and C) or anti-RT MAb (B and D).