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. 2024 May 29;43(14):3009–3026. doi: 10.1038/s44318-024-00128-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) CDMS of native C4BP, (B) MP of native C4BP, (C) CDMS of acidified C4BP, (D) MP of acidified C4BP. Native C4BP (A, B) exhibited two major populations corresponding to α7β1 + ProS (dark blue) and overlapping α6β1+ProS (light blue)/α7β1 (dark orange), as well as a small portion of unbound ProS (green) in MP. The C4BP samples, obtained by acidification (C, D), show ProS dissociation (green), enable isoform differentiation, and highlight dominant C4BP(β+) variants. Bars in (C, D) show isoform abundance of α7β1 (blue) and α6β1 (orange) in the sample, as quantified for denatured CDMS and MP, respectively. Of note, both CDMS and MP were optimized for the detection of the C4BP assembly (~400–800 kDa). Therefore, the quantification of lower MW species, such as monomeric ProS, is more ambiguous. Source data are available online for this figure.