Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jun 3;16(7):1495–1514. doi: 10.1038/s44321-024-00084-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

Figure EV3 — (A) Schematic representation of the STAPL-3D pipeline with key optimization steps for single organoid segmentation. (B) Representative raw 3D imaging data and associated 3D rendered single organoid segmentation. (C, D) Violin plots of the mean fluorescent intensities (MFI) of the screened probes for all individual organoids from the NB (C) and BC (D) PDO biobanks normalized for the experimental day and probe. Dashed line indicates the cut-off used for considering an organoid as positively stained. Boxplots inside violin plots; centre: median, bounds: Q1–Q3, whiskers extend to minimum/maximum limited to 1.5 times the IQR. n = 3 independent experiments. (E, F) Representation of the spatial target distribution used for UMAP clustering of both the NB (E) and BC (F) PDO biobank. n = 3 independent experiments.