Fig. 5.

hsa-miR-20a-5p and hsa-miR-106b-5p regulate the RORA gene and protein expression in PCa cells. A Study of RORA and B miRNAs expression in histologically normal prostate epithelial cell line (RWPE-1) and the androgen-sensitive PCa cell line (22-Rv1). C RORA gene expression levels in 22Rv1 transfected with siRORA, miR-20a-5p inhibitor (i20a-5p) or miR-106b-5p inhibitor (i106b-5p) and co-transfected with siRORA and either i20a-5p or i106-5p. D RORA protein expression in 22Rv1 cells transfected with i20a-5p or i106b-5p inhibitor. A representative Western blot is presented (top). The membranes were tested with the corresponding antibodies. Expression levels were normalized to β-actin, then miRNAs expression levels were normalized to negative control inhibitor (iNC). Full-length blot and gel are presented in Additional File 8: Figure S6. Working concentration for siRORA was 50 nM, and for i20a-5p or i106b-5p was 15nM. Symbols: * indicates significant differences, *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001