FIG. 3.

Effects of HCV core production on NF-κB activity with and without treatment with anti-Fas or TNF-α. HepG2 cells were transfected with 0.5 μg of pNF-κB-Luc and 1.5 μg of various expression plasmids with 3 μl of FuGENE 6. At 46 h posttransfection, cells were treated or not with anti-Fas (100 ng/ml) or TNF-α (10 ng/ml) for 2 h as indicated. A whole-cell lysate was prepared and assayed for luciferase activity. The data represent the means of the relative luciferase activities in three independent experiments.